Substrate specificity of methylthioadenosine phosphorylase from human liver.
نویسندگان
چکیده
منابع مشابه
Substrate specificity of 5'-methylthioadenosine phosphorylase from human prostate.
5'-Methylthioadenosine phosphorylase was purified approx. 340-fold from human prostate by using affinity chromatography by Hg-coupled Sepharose. The enzyme, responsible for the breakdown of 5'-methylthioadenosine into adenine and methylthioribose 1-phosphate, was partially characterized. The apparent Km for 5'-methylthioadenosine is 25 microM. It is activated by thiols and shows an absolute req...
متن کاملAbsence of Methylthioadenosine Phosphorylase in Human Gliomas1
All normal mammalian tissues contain methylthioadenosine phosphorylase, which plays a role in the recycling of purines and methionine consumed during polyamine synthesis. A complete deficiency of methyl thioadenosine phosphor) lase has been reported in some human leukemias and lymphomas and in a few solid tumors. The exact incidence of the enzyme deficiency among fresh human tumor specimens has...
متن کاملExpression and Function of Methylthioadenosine Phosphorylase in Chronic Liver Disease
UNLABELLED To study expression and function of methylthioadenosine phosphorylase (MTAP), the rate-limiting enzyme in the methionine and adenine salvage pathway, in chronic liver disease. DESIGN MTAP expression was analyzed by qRT-PCR, Western blot and immunohistochemical analysis. Levels of MTA were determined by liquid chromatography-tandem mass spectrometry. RESULTS MTAP was downregulated...
متن کاملTherapeutic targeting of methylthioadenosine phosphorylase
Methylthioadenosine Phosphorylase is a well-known tumor suppressor and a regulator for purine and pyrimidine synthesis and metabolism. Several previous studies show MTAP could be a prognostic marker independent or coordinate with p16 in multiple cancer types. Furthermore, inhibitors of MTAP have been developed and tested in in vitro and in vivo experiment to support the selective tumor cell-kil...
متن کاملHomozygous deletions of methylthioadenosine phosphorylase in human biliary tract cancers.
The p16(INK4A)/CDKN2A gene on chromosome 9p21 is a site of frequent allelic loss in human cancers, and in a subset of cases, homozygous deletions at this locus encompass the telomeric methylthioadenosine phosphorylase (MTAP) gene. The MTAP gene product is the principal enzyme involved in purine synthesis via the salvage pathway, such that MTAP-negative cancers are solely dependent on de novo pu...
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ژورنال
عنوان ژورنال: Acta Biochimica Polonica
سال: 1994
ISSN: 1734-154X,0001-527X
DOI: 10.18388/abp.1994_4683